Human EIF2AK3 (NP_004827.3, 536 a.a. - 1116 a.a.) partial recombinant protein with GST tag expressed in Escherichia coli.
Theoretical MW (kDa):
Escherichia coli expression system
Glutathione sepharose chromatography
51 % by SDS-PAGE/CBB staining
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: SRPKtide. ATP: 100 uM.
Quality Control Testing:
Loading 1 ug protein in SDS-PAGE
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.