PDK2 (Human) Recombinant Protein
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Specification
Product Description
Human PDK2 (NP_002602.2, 1 a.a. - 407 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
73
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
77 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: PDHKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PDK2
Entrez GeneID
5164Protein Accession#
NP_002602.2Gene Name
PDK2
Gene Alias
PDHK2
Gene Description
pyruvate dehydrogenase kinase, isozyme 2
Omim ID
602525Gene Ontology
HyperlinkGene Summary
O
Other Designations
pyruvate dehydrogenase kinase, isoenzyme 2
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