PASK (Human) Recombinant Protein
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Specification
Product Description
Human PASK (NP_055963.2, 949 a.a. - 1323 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
69
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
74 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: GS peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PASK
Entrez GeneID
23178Protein Accession#
NP_055963.2Gene Name
PASK
Gene Alias
DKFZp434O051, DKFZp686P2031, KIAA0135, PASKIN, STK37
Gene Description
PAS domain containing serine/threonine kinase
Omim ID
607505Gene Ontology
HyperlinkGene Summary
PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PASK is an evolutionarily conserved protein present in yeast, flies, and mammals.[supplied by OMIM
Other Designations
PAS-serine/threonine kinase
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Interactome
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