OXSR1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human OXSR1 (NP_005100.1, 1 a.a. - 527 a.a.) full-length recombinant protein with GST tag co-expressed with His-tagged WNK1 (NP_061852.1, 1 a.a. - 491 a.a.) expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
85
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
98 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated peptide in streptavidin-coated ELISA plate. Phosphorylation was detected by HRP-labeled anti-phospho antibody. Substrate: CATCHtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — OXSR1
Entrez GeneID
9943Protein Accession#
NP_005100.1Gene Name
OXSR1
Gene Alias
KIAA1101, OSR1
Gene Description
oxidative-stress responsive 1
Omim ID
604046Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Epinephrine inhibits PI3Kα via the Hippo kinases.
Ting-Yu Lin, Shakti Ramsamooj, Tiffany Perrier, Katarina Liberatore, Louise Lantier, Neil Vasan, Kannan Karukurichi, Seo-Kyoung Hwang, Edward A Kesicki, Edward R Kastenhuber, Thorsten Wiederhold, Tomer M Yaron, Emily M Huntsman, Mengmeng Zhu, Yilun Ma, Marcia N Paddock, Guoan Zhang, Benjamin D Hopkins, Owen McGuinness, Robert E Schwartz, Baran A Ersoy, Lewis C Cantley, Jared L Johnson, Marcus D Goncalves.
Cell Reports 2023 Dec; 42(12):113535.
Application：Thin-layer chromatography, Human, Recombinant protein .
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Epinephrine inhibits PI3Kα via the Hippo kinases.
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