NEK9 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human NEK9 (NP_149107.3, 1 a.a. - 346, 733 a.a. - 979 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
93
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography and anion exchange chromatography
Purity
87 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CDK7 peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — NEK9
Entrez GeneID
91754Protein Accession#
NP_149107.3Gene Name
NEK9
Gene Alias
DKFZp434D0935, MGC138306, MGC16714, NERCC, NERCC1, Nek8
Gene Description
NIMA (never in mitosis gene a)- related kinase 9
Omim ID
609798Gene Ontology
HyperlinkOther Designations
NIMA related kinase 9|NIMA-related kinase Nek8
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