RPS6KA4 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human RPS6KA4 (NP_003933.1, 1 a.a. - 772 a.a.) full-length recombinant protein with GST tag co-expressed with His-tagged MAPK1 (NP_002736.3, 1 a.a. - 360 a.a.) expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
114
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
76 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Crosstide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — RPS6KA4
Entrez GeneID
8986Protein Accession#
NP_003933.1Gene Name
RPS6KA4
Gene Alias
MSK2, RSK-B
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 4
Omim ID
603606Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
mitogen- and stress-activated protein kinase 2|ribosomal protein S6 kinase alpha 4|ribosomal protein S6 kinase, 90kD, polypeptide 4|ribosomal protein kinase B
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