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RPS6KA4 (Human) Recombinant ProteinBioActive

  • Catalog # : P5604
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  • Specification
  • Product Description:
  • Human RPS6KA4 (NP_003933.1, 1 a.a. - 772 a.a.) full-length recombinant protein with GST tag co-expressed with His-tagged MAPK1 (NP_002736.3, 1 a.a. - 360 a.a.) expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 114
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 76 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Crosstide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5604
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 8986
  • Gene Name:
  • RPS6KA4
  • Gene Alias:
  • MSK2,RSK-B
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 4
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
  • Other Designations:
  • mitogen- and stress-activated protein kinase 2,ribosomal protein S6 kinase alpha 4,ribosomal protein S6 kinase, 90kD, polypeptide 4,ribosomal protein kinase B
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