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CDC42BPA (Human) Recombinant ProteinBioActive

  • Catalog # : P5603
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  • Specification
  • Product Description:
  • Human CDC42BPA (NP_003598.2, 1 a.a. - 574 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 93
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 97 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate:DAPK1tide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5603
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 8476
  • Gene Name:
  • CDC42BPA
  • Gene Alias:
  • DKFZp686L1738,DKFZp686P1738,FLJ23347,KIAA0451,MRCK,MRCKA,PK428
  • Gene Description:
  • CDC42 binding protein kinase alpha (DMPK-like)
  • Gene Summary:
  • The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq
  • Other Designations:
  • CDC42 binidng protein kinase beta,CDC42-binding protein kinase alpha,CDC42-binding protein kinase alpha (DMPK-like),OTTHUMP00000035727,OTTHUMP00000035728,myotonic dystrophy kinase-related CDC42-binding protein kinase alpha,ser-thr protein kinase PK428,ser
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