CDC42BPA (Human) Recombinant Protein
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Specification
Product Description
Human CDC42BPA (NP_003598.2, 1 a.a. - 574 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
93
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
97 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate:DAPK1tide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CDC42BPA
Entrez GeneID
8476Protein Accession#
NP_003598.2Gene Name
CDC42BPA
Gene Alias
DKFZp686L1738, DKFZp686P1738, FLJ23347, KIAA0451, MRCK, MRCKA, PK428
Gene Description
CDC42 binding protein kinase alpha (DMPK-like)
Omim ID
603412Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq
Other Designations
CDC42 binidng protein kinase beta|CDC42-binding protein kinase alpha|CDC42-binding protein kinase alpha (DMPK-like)|OTTHUMP00000035727|OTTHUMP00000035728|myotonic dystrophy kinase-related CDC42-binding protein kinase alpha|ser-thr protein kinase PK428|ser
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Interactome
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Disease
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