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MAP3K11 (Human) Recombinant ProteinBioActive

  • Catalog # : P5600
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  • Specification
  • Product Description:
  • Human MAP3K11 (NP_002410.1, 99 a.a. - 398 a.a.) partial recombinant protein with His tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 37
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Ni-NTA affinity chromatography
  • Purity:
  • 81 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5600
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 4296
  • Gene Name:
  • MAP3K11
  • Gene Alias:
  • MGC17114,MLK-3,MLK3,PTK1,SPRK
  • Gene Description:
  • mitogen-activated protein kinase kinase kinase 11
  • Gene Summary:
  • The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq
  • Other Designations:
  • SH3 domain-containing proline-rich kinase,mixed lineage kinase 3,protein-tyrosine kinase PTK1
  • Gene Pathway
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