MAP3K9 (Human) Recombinant Protein
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Specification
Product Description
Human MAP3K9 (NP_149132.2, 110 a.a. - 422 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
62
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
96 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MAP3K9
Entrez GeneID
4293Protein Accession#
NP_149132.2Gene Name
MAP3K9
Gene Alias
MLK1, PRKE1
Gene Description
mitogen-activated protein kinase kinase kinase 9
Omim ID
600136Gene Ontology
HyperlinkOther Designations
mixed lineage kinase 1 (tyr and ser/thr specificity)
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Interactome
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Pathway
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Disease
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