MGC42105 (Human) Recombinant Protein
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Specification
Product Description
Human MGC42105 (NP_699192.1, 1 a.a. - 436 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
76
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography and anion exchange chromatography
Purity
71 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: CHK1tide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MGC42105
Entrez GeneID
167359Protein Accession#
NP_699192.1Gene Name
MGC42105
Gene Alias
NIM1
Gene Description
serine/threonine-protein kinase NIM1
Gene Ontology
HyperlinkOther Designations
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Disease
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