MARK4 (Human) Recombinant Protein
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Specification
Product Description
Human MARK4 (NP_113605.2, 1 a.a. - 688 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
103
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography and anion exchange chromatography
Purity
85 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CHKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MARK4
Entrez GeneID
57787Protein Accession#
NP_113605.2Gene Name
MARK4
Gene Alias
FLJ90097, KIAA1860, MARKL1, Nbla00650
Gene Description
MAP/microtubule affinity-regulating kinase 4
Omim ID
606495Gene Ontology
HyperlinkOther Designations
MAP/microtubule affinity-regulating kinase like 1|MARK4 serine/threonine protein kinase|putative protein product of Nbla00650
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