MAP3K4 (Human) Recombinant Protein
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Specification
Product Description
Human MAP3K4 (NP_005913.2, 1312 a.a. - 1608 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
61
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
91 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG). Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MAP3K4
Entrez GeneID
4216Protein Accession#
NP_005913.2Gene Name
MAP3K4
Gene Alias
FLJ42439, KIAA0213, MAPKKK4, MEKK4, MTK1, PRO0412
Gene Description
mitogen-activated protein kinase kinase kinase 4
Omim ID
602425Gene Ontology
HyperlinkGene Summary
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 4|MAPK/ERK kinase kinase 4|SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of|dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4)|predicted protein of HQ0412
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Interactome
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Pathway
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Disease
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Publication Reference
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MEKK4-mediated Phosphorylation of HOXA10 at Threonine 362 facilitates embryo adhesion to the endometrial epithelium.
Mei Zhang, Qun Zhang, Zhiwen Cao, Xinyu Cai, Jingyu Liu, Yue Jiang, Yingchun Zhu, Jidong Zhou, Lina Yu, Xin Zhen, Yali Hu, Guijun Yan, Haixiang Sun.
Cell Death Discovery 2022 Oct; 8(1):415.
Application:KA, Recombinant proteins.
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MEKK4-mediated Phosphorylation of HOXA10 at Threonine 362 facilitates embryo adhesion to the endometrial epithelium.
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