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STK11/CAB39/STRADA (Human) Recombinant ProteinBioActive

  • Catalog # : P5577
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  • Countries :
  • Specification
  • Product Description:
  • Human STK11 (NP_000446.1, 1 a.a. - 433 a.a.) and CAB39 (NP_057373.1, 1 a.a. - 341 a.a.) and STRADA (NP_001003787.1, 1 a.a. - 431 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 75
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 97 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein/peptide, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin-coated plate. Phosphorylation was detected by anti- phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: LKBtide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5577
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 6794
  • Protein Accession#:
  • NP_000446.1 (Gene ID : 6794);NP_057373.1 (Gene ID : 51719);NP_001003787.1 (Gene ID : 92335)
  • Gene Name:
  • STK11
  • Gene Alias:
  • LKB1,PJS
  • Gene Description:
  • serine/threonine kinase 11
  • Gene Summary:
  • This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
  • Other Designations:
  • polarization-related protein LKB1,serine/threonine protein kinase 11
  • Gene Information
  • Protein Accession#:
  • NP_000446.1 (Gene ID : 6794);NP_057373.1 (Gene ID : 51719);NP_001003787.1 (Gene ID : 92335)
  • Gene Name:
  • CAB39
  • Gene Alias:
  • CGI-66,FLJ22682,MO25
  • Gene Description:
  • calcium binding protein 39
  • Other Designations:
  • -
  • Gene Information
  • Protein Accession#:
  • NP_000446.1 (Gene ID : 6794);NP_057373.1 (Gene ID : 51719);NP_001003787.1 (Gene ID : 92335)
  • Gene Name:
  • STRADA
  • Gene Alias:
  • FLJ90524,LYK5,NY-BR-96,PMSE,STRAD,Stlk
  • Gene Description:
  • STE20-related kinase adaptor alpha
  • Gene Summary:
  • The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq
  • Other Designations:
  • STE20-like pseudokinase,STE20-related adaptor protein,protein kinase LYK5
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