STK11/CAB39/STRADA (Human) Recombinant Protein
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Specifications
Product Description
Human STK11 (NP_000446.1, 1 a.a. - 433 a.a.) and CAB39 (NP_057373.1, 1 a.a. - 341 a.a.) and STRADA (NP_001003787.1, 1 a.a. - 431 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
75
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
97 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein/peptide, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin-coated plate. Phosphorylation was detected by anti- phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: LKBtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — STK11
Entrez GeneID
6794Protein Accession#
NP_000446.1 (Gene ID : 6794);NP_057373.1 (Gene ID : 51719);NP_001003787.1 (Gene ID : 92335)Gene Name
STK11
Gene Alias
LKB1, PJS
Gene Description
serine/threonine kinase 11
Gene Ontology
HyperlinkGene Summary
This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
polarization-related protein LKB1|serine/threonine protein kinase 11
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Gene Info — CAB39
Entrez GeneID
51719Protein Accession#
NP_000446.1 (Gene ID : 6794);NP_057373.1 (Gene ID : 51719);NP_001003787.1 (Gene ID : 92335)Gene Name
CAB39
Gene Alias
CGI-66, FLJ22682, MO25
Gene Description
calcium binding protein 39
Gene Ontology
HyperlinkOther Designations
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Gene Info — STRADA
Entrez GeneID
92335Protein Accession#
NP_000446.1 (Gene ID : 6794);NP_057373.1 (Gene ID : 51719);NP_001003787.1 (Gene ID : 92335)Gene Name
STRADA
Gene Alias
FLJ90524, LYK5, NY-BR-96, PMSE, STRAD, Stlk
Gene Description
STE20-related kinase adaptor alpha
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq
Other Designations
STE20-like pseudokinase|STE20-related adaptor protein|protein kinase LYK5
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