IRAK1 (Human) Recombinant Protein
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Specification
Product Description
Human IRAK1 (NP_001560.2, 194 a.a. - 712 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
83
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
100 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: SRPKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IRAK1
Entrez GeneID
3654Protein Accession#
NP_001560.2Gene Name
IRAK1
Gene Alias
IRAK, pelle
Gene Description
interleukin-1 receptor-associated kinase 1
Omim ID
300283Gene Ontology
HyperlinkGene Summary
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026014|OTTHUMP00000026020|Pelle homolog
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