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CHUK (Human) Recombinant ProteinBioActive

  • Catalog # : P5564
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  • Specification
  • Product Description:
  • Human CHUK (NP_001269.3, 1 a.a. - 745 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 111
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 93 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: I-kappaB-alpha peptide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5564
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1147
  • Gene Name:
  • CHUK
  • Gene Alias:
  • IKBKA,IKK-alpha,IKK1,IKKA,NFKBIKA,TCF16
  • Gene Description:
  • conserved helix-loop-helix ubiquitous kinase
  • Gene Summary:
  • This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq
  • Other Designations:
  • I-kappa-B kinase 1,I-kappa-B kinase-alpha,IKK-a kinase,IkB kinase alpha subunit,Nuclear factor NFkappaB inhibitor kinase alpha,OTTHUMP00000020273,conserved helix-loop ubiquitous kinase
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