ICK (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Human ICK (NP_057597.2, 1 a.a. - 632 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
98
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
74 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Modified Erktide. ATP: 1000 uM
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
-
Applications
Functional Study
SDS-PAGE
-
Gene Info — ICK
Entrez GeneID
22858Protein Accession#
NP_057597.2Gene Name
ICK
Gene Alias
KIAA0936, LCK2, MGC46090, MRK
Gene Description
intestinal cell (MAK-like) kinase
Gene Ontology
HyperlinkGene Summary
Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq
Other Designations
MAK-related kinase|OTTHUMP00000016630|OTTHUMP00000039961|intestinal cell kinase|serine/threonine protein kinase
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com