TNNI3K (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human TNNI3K (NP_057062.1, 1 a.a. - 727 a.a.) partial recombinant protein with His tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
108
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
97 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated peptide in streptavidin-coated ELISA plate. Phosphorylation was detected by HRP-labeled anti-phospho antibody. Substrate: Poly-(GT). ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TNNI3K
Entrez GeneID
51086Protein Accession#
NP_057062.1Gene Name
TNNI3K
Gene Alias
CARK, MGC142099, MGC33828
Gene Description
TNNI3 interacting kinase
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000011170|cardiac ankyrin repeat kinase
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Interactome
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Disease
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