GSG2 (Human) Recombinant Protein
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Specification
Product Description
Human GSG2 (NP_114171.2, 1 a.a. - 798 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
116
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
73 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Histone H3 peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — GSG2
Entrez GeneID
83903Protein Accession#
NP_114171.2Gene Name
GSG2
Gene Alias
HASPIN
Gene Description
germ cell associated 2 (haspin)
Omim ID
609240Gene Ontology
HyperlinkOther Designations
OTTHUMP00000115873|germ cell-specific gene 2|haploid germ cell-specific nuclear protein kinase|haspin
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