EIF2AK4 (Human) Recombinant Protein
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Specification
Product Description
Human EIF2AK4 (NP_001013725, 1 a.a. - 1649 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
215
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
90 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein and tRNA, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti- phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: EIF2S1. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — EIF2AK4
Entrez GeneID
440275Protein Accession#
NP_001013725; Q9P2K8Gene Name
EIF2AK4
Gene Alias
GCN2, KIAA1338
Gene Description
eukaryotic translation initiation factor 2 alpha kinase 4
Omim ID
609280Gene Ontology
HyperlinkGene Summary
EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM
Other Designations
GCN2 eIF2alpha kinase
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Interactome
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Publication Reference
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GCN2 kinase activation by ATP-competitive kinase inhibitors.
Colin P Tang, Owen Clark, John R Ferrarone, Carl Campos, Alshad S Lalani, John D Chodera, Andrew M Intlekofer, Olivier Elemento, Ingo K Mellinghoff.
Nature Chemical Biology 2022 Feb; 18(2):207.
Application:KA, Human, Recombinant proteins.
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TIPRL potentiates survival of lung cancer by inducing autophagy through the eIF2α-ATF4 pathway.
Jeon SJ, Ahn JH, Halder D, Cho HS, Lim JH, Jun SY, Lee JJ, Yoon JY, Choi MH, Jung CR, Kim JM, Kim NS.
Cell Death & Disease 2019 Dec; 10(12):959.
Application:KA, Human, 293T cells.
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GCN2 kinase activation by ATP-competitive kinase inhibitors.
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