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EPHA8 (Human) Recombinant ProteinBioActive

  • Catalog # : P5547
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  • Specification
  • Product Description:
  • Human EPHA8 (NP_065387.1, 571 a.a. - 924 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 67
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 89 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Blk/Lyntide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5547
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 2046
  • Gene Name:
  • EPHA8
  • Gene Alias:
  • EEK,HEK3,KIAA1459
  • Gene Description:
  • EPH receptor A8
  • Gene Summary:
  • This gene encodes a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The protein encoded by this gene functions as a receptor for ephrin A2, A3 and A5 and plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. [provided by RefSeq
  • Other Designations:
  • EPH- and ELK-related tyrosine kinase,OTTHUMP00000002934,OTTHUMP00000002935,ephrin type-A receptor 8,hydroxyaryl-protein kinase,protein-tyrosine kinase,tyrosine-protein kinase receptor EEK,tyrosylprotein kinase
  • Gene Pathway
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