EPHA6 (Human) Recombinant Protein
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Specification
Product Description
Human EPHA6 (CAD38242.1, 683 a.a. - 1130 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
77
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
84 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Blk/Lyntide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — EPHA6
Entrez GeneID
285220Protein Accession#
CAD38242.1Gene Name
EPHA6
Gene Alias
DKFZp434C1418, EPA6, FLJ35246, PRO57066
Gene Description
EPH receptor A6
Gene Ontology
HyperlinkOther Designations
ephrin receptor EphA6
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Pathway
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Disease
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