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EEF2K (Human) Recombinant ProteinBioActive

  • Catalog # : P5544
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  • Specification
  • Product Description:
  • Human EEF2K (NP_037434.1, 1 a.a. - 725 a.a.) full-length recombinant protein with GST tag expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 109
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 81 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: EEF2Ktide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5544
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • EEF2K
  • Gene Alias:
  • HSU93850,MGC45041,eEF-2K
  • Gene Description:
  • eukaryotic elongation factor-2 kinase
  • Gene Summary:
  • This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq
  • Other Designations:
  • calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase,calmodulin-dependent protein kinase III,eEF-2 kinase,elongation factor-2 kinase
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