STK17A (Human) Recombinant Protein
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Specification
Product Description
Human STK17A (NP_004751.2, 1 a.a. - 414 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
74
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
92 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: ZIPtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — STK17A
Entrez GeneID
9263Protein Accession#
NP_004751.2Gene Name
STK17A
Gene Alias
DRAK1
Gene Description
serine/threonine kinase 17a
Omim ID
604726Gene Ontology
HyperlinkGene Summary
This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq
Other Designations
DAP kinase-related apoptosis-inducing protein kinase 1|death-associated protein kinase-related 1|serine/threonine kinase 17a (apoptosis-inducing)
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Interactome
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Disease
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