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DCLK1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5532
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  • Specification
  • Product Description:
  • Human DCLK1 (NP_004725.1, 1 a.a. - 729 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 108
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 58 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: GS peptide. ATP: 1000 uM
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5532
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 9201
  • Gene Name:
  • DCLK1
  • Gene Alias:
  • DCAMKL1,DCDC3A,DCLK,KIAA0369
  • Gene Description:
  • doublecortin-like kinase 1
  • Gene Summary:
  • DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000018250,doublecortin and CaM kinase-like 1
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