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CSNK2A1/CSNK2B (Human) Recombinant ProteinBioActive

  • Catalog # : P5526
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  • Specification
  • Product Description:
  • Human CSNK2A1 (NP_001886.1, , 1 a.a. - 391 a.a.) and CSNK2B (NP_001311.3, 1 a.a. - 215 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 72
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 98 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5526
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1457
  • Protein Accession#:
  • NP_001886.1 (Gene ID : 1457);NP_001311.3 (Gene ID : 1460)
  • Gene Name:
  • CSNK2A1
  • Gene Alias:
  • CK2A1,CKII
  • Gene Description:
  • casein kinase 2, alpha 1 polypeptide
  • Gene Summary:
  • Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CK2 catalytic subunit alpha,OTTHUMP00000029925,OTTHUMP00000029926,casein kinase II alpha 1 subunit,casein kinase II alpha subunit,protein kinase CK2
  • Gene Information
  • Entrez GeneID:
  • 1460
  • Protein Accession#:
  • NP_001886.1 (Gene ID : 1457);NP_001311.3 (Gene ID : 1460)
  • Gene Name:
  • CSNK2B
  • Gene Alias:
  • CK2B,CK2N,CSK2B,G5A,MGC138222,MGC138224
  • Gene Description:
  • casein kinase 2, beta polypeptide
  • Gene Summary:
  • This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq
  • Other Designations:
  • Casein kinase II beta subunit,OTTHUMP00000029060,OTTHUMP00000029063,OTTHUMP00000029064,alternative name: G5a, phosvitin,phosvitin
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