CSNK1D (Human) Recombinant Protein
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Specification
Product Description
Human CSNK1D (NP_001884.2, 1 a.a. - 294 a.a.) partial recombinant protein with GST tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
61
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Glutathione sepharose chromatography
Purity
64 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSNK1D
Entrez GeneID
1453Protein Accession#
NP_001884.2Gene Name
CSNK1D
Gene Alias
HCKID
Gene Description
casein kinase 1, delta
Omim ID
600864Gene Ontology
HyperlinkGene Summary
This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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