CSNK1G2 (Human) Recombinant Protein
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Specification
Product Description
Human CSNK1G2 (NP_001310.3, 1 a.a. - 415 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
74
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
95 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSNK1G2
Entrez GeneID
1455Protein Accession#
NP_001310.3Gene Name
CSNK1G2
Gene Alias
CK1g2
Gene Description
casein kinase 1, gamma 2
Omim ID
602214Gene Ontology
HyperlinkGene Summary
O
Other Designations
casein kinase 1 isoform gamma-2
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Interactome
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Pathway
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