PRKG2 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PRKG2 (NP_006250.1, 1 a.a. - 762 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
114
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
74 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and cGMP. The phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: PKAtide3. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PRKG2
Entrez GeneID
5593Protein Accession#
NP_006250.1Gene Name
PRKG2
Gene Alias
PRKGR2, cGKII
Gene Description
protein kinase, cGMP-dependent, type II
Omim ID
601591Gene Ontology
HyperlinkGene Summary
cGMP-dependent
Other Designations
OTTHUMP00000160807
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Interactome
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Pathway
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Disease
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