CDK8/CCNC (Human) Recombinant Protein
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Specification
Product Description
Human CDK8 (NP_001251.1, 1 a.a. - 464 a.a.) and CCNC (NP_005181.2, 1 a.a. - 283 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
80
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
91 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: RNA polymerase peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CCNC
Entrez GeneID
892Protein Accession#
NP_001251.1 (Gene ID : 1024);NP_005181.2 (Gene ID : 892)Gene Name
CCNC
Gene Alias
CycC
Gene Description
cyclin C
Omim ID
123838Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016897
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Gene Info — CDK8
Entrez GeneID
1024Protein Accession#
NP_001251.1 (Gene ID : 1024);NP_005181.2 (Gene ID : 892)Gene Name
CDK8
Gene Alias
K35, MGC126074, MGC126075
Gene Description
cyclin-dependent kinase 8
Omim ID
603184Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq
Other Designations
CDK8 protein kinase|OTTHUMP00000018158|cell division protein kinase 8|protein kinase K35
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