CDK7/CCNH/MNAT1 (Human) Recombinant Protein
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Specification
Product Description
Human CDK7 (NP_001790.1, 1 a.a. - 346 a.a.) and CCNH (NP_001230.1, 1 a.a. - 323 a.a.) and MNAT1 (NP_002422.1, 1 a.a. - 309 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
66
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
99 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CTD3 peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CCNH
Entrez GeneID
902Protein Accession#
NP_001790.1 (Gene ID : 1022);NP_001230.1 (Gene ID : 902);NP_002422.1 (Gene ID : 4331)Gene Name
CCNH
Gene Alias
CAK, p34, p37
Gene Description
cyclin H
Omim ID
601953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. [provided by RefSeq
Other Designations
CDK-activating kinase|MO15-associated protein|cyclin-dependent kinase-activating kinase
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Gene Info — CDK7
Entrez GeneID
1022Protein Accession#
NP_001790.1 (Gene ID : 1022);NP_001230.1 (Gene ID : 902);NP_002422.1 (Gene ID : 4331)Gene Name
CDK7
Gene Alias
CAK1, CDKN7, MO15, STK1, p39MO15
Gene Description
cyclin-dependent kinase 7
Omim ID
601955Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq
Other Designations
39 KDa protein kinase|Cdk-activating kinase|cell division protein kinase 7|cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)|homolog of Xenopus MO15 Cdk-activating kinase|kinase subunit of CAK|serine/threonine kinase stk1|ser
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Gene Info — MNAT1
Entrez GeneID
4331Protein Accession#
NP_001790.1 (Gene ID : 1022);NP_001230.1 (Gene ID : 902);NP_002422.1 (Gene ID : 4331)Gene Name
MNAT1
Gene Alias
MAT1, RNF66, TFB3
Gene Description
menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)
Omim ID
602659Gene Ontology
HyperlinkGene Summary
Cyclin-dependent kinases (CDKs), which play an essential role in cell cycle control of eukaryotic cells, are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 (MIM 601955), cyclin H (CCNH; MIM 601953), and MAT1. MAT1 (for 'menage a trois-1') is involved in the assembly of the CAK complex.[supplied by OMIM
Other Designations
cyclin G1 interacting protein|cyclin H assembly factor|menage a trois 1 (CAK assembly factor)
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Interactome
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Pathway
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Disease
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