CDC7/DBF4 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CDC7 (NP_003494.1, 1 a.a. - 574 a.a.) and DBF4 (NP_006707.1, 1 a.a. - 674 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
92
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
60 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: MCM2 peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CDC7
Entrez GeneID
8317Protein Accession#
NP_003494.1 (Gene ID : 8317);NP_006707.1 (Gene ID : 10926)Gene Name
CDC7
Gene Alias
CDC7L1, HsCDC7, Hsk1, MGC117361, MGC126237, MGC126238, huCDC7
Gene Description
cell division cycle 7 homolog (S. cerevisiae)
Omim ID
603311Gene Ontology
HyperlinkGene Summary
This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq
Other Designations
CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1|OTTHUMP00000011788|cell division cycle 7|cell division cycle 7-like protein 1
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Gene Info — DBF4
Entrez GeneID
10926Protein Accession#
NP_003494.1 (Gene ID : 8317);NP_006707.1 (Gene ID : 10926)Gene Name
DBF4
Gene Alias
ASK, CHIF, DBF4A, ZDBF1
Gene Description
DBF4 homolog (S. cerevisiae)
Omim ID
604281Gene Ontology
HyperlinkGene Summary
DBF-type containing 1
Other Designations
OTTHUMP00000161661|activator of S phase kinase|chiffon homolog|zinc finger, DBF-type containing 1
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Interactome
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Pathway
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Disease
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