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CDC7/DBF4 (Human) Recombinant ProteinBioActive

  • Catalog # : P5510
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  • Specification
  • Product Description:
  • Human CDC7 (NP_003494.1, 1 a.a. - 574 a.a.) and DBF4 (NP_006707.1, 1 a.a. - 674 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 92
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 60 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: MCM2 peptide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5510
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 8317
  • Protein Accession#:
  • NP_003494.1 (Gene ID : 8317);NP_006707.1 (Gene ID : 10926)
  • Gene Name:
  • CDC7
  • Gene Alias:
  • CDC7L1,HsCDC7,Hsk1,MGC117361,MGC126237,MGC126238,huCDC7
  • Gene Description:
  • cell division cycle 7 homolog (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq
  • Other Designations:
  • CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1,OTTHUMP00000011788,cell division cycle 7,cell division cycle 7-like protein 1
  • Gene Information
  • Protein Accession#:
  • NP_003494.1 (Gene ID : 8317);NP_006707.1 (Gene ID : 10926)
  • Gene Name:
  • DBF4
  • Gene Alias:
  • ASK,CHIF,DBF4A,ZDBF1
  • Gene Description:
  • DBF4 homolog (S. cerevisiae)
  • Other Designations:
  • OTTHUMP00000161661,activator of S phase kinase,chiffon homolog,zinc finger, DBF-type containing 1
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