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CDC2/CCNB1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5508
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  • Specification
  • Product Description:
  • Human CDC2 (NP_001777.1, 1 a.a. - 297 a.a.) and CCNB1 (NP_114172.1, 1 a.a. - 433 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 61
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 58 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: CDK1S1 peptide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5508
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 983
  • Protein Accession#:
  • NP_001777.1 (Gene ID : 983);NP_114172.1 (Gene ID : 891)
  • Gene Name:
  • CDC2
  • Gene Alias:
  • CDC28A,CDK1,DKFZp686L20222,MGC111195
  • Gene Description:
  • cell division cycle 2, G1 to S and G2 to M
  • Gene Summary:
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase
  • Gene Information
  • Entrez GeneID:
  • 891
  • Protein Accession#:
  • NP_001777.1 (Gene ID : 983);NP_114172.1 (Gene ID : 891)
  • Gene Name:
  • CCNB1
  • Gene Alias:
  • CCNB
  • Gene Description:
  • cyclin B1
  • Gene Summary:
  • The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
  • Other Designations:
  • G2/mitotic-specific cyclin B1
  • Interactome
  • Interactome
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