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CAMK2B (Human) Recombinant ProteinBioActive

  • Catalog # : P5504
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  • Specification
  • Product Description:
  • Human CAMK2B (NP_742078.1, 1 a.a. - 503 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 83
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 74 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: GS peptide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5504
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 816
  • Gene Name:
  • CAMK2B
  • Gene Alias:
  • CAM2,CAMK2,CAMKB,MGC29528
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II beta
  • Gene Summary:
  • The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • CaM kinase II beta subunit,CaM-kinase II beta chain,CaMK-II beta subunit,OTTHUMP00000159355,OTTHUMP00000159356,calcium/calmodulin-dependent protein kinase (CaM kinase) II beta,calcium/calmodulin-dependent protein kinase type II beta chain,proline rich cal
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