Human TNFSF10 extracellular domain (91 a.a. - 281 a.a.) partial recombinant protein expressed in Escherichia coli and purified to homogeneity as a homotrimer.
MILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG The first M is partially removed.
Theoretical MW (kDa):
Escherichia coli expression system
< 0.1 EU/ug as determined by Endo-Safe LAL Assay.
The biological activity of TNFSF10 is measured by its ability to induce cell death to A172 human glioblastoma cell line.
Quality Control Testing:
Loading 5 ug protein in SDS-PAGE
In 25 mM HEPES, pH7.5, 150 mM NaCl, 5 mM DTT.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Result of activity analysis
TNFSF10 induce cell death to A172 human glioblastoma cell line.
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. [provided by RefSeq