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PAK7 (Human) Recombinant ProteinBioActive

  • Catalog # : P5385
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  • Specification
  • Product Description:
  • Human PAK7 kinase domain (Q9P286, 425 a.a. - 719 a.a.) partial recombinant protein expressed in Escherichia coli.
  • Sequence:
  • GPHMSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAIRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH
    The first 4 residues GPHM are from Turbo3C Protease cleavage site. The underlined S is phosphorylated S602.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 33.9
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Concentration:
  • 1 mg/ml
  • Activity:
  • Specific activity: 4,199 pmoles/min/ug
  • Quality Control Testing:
  • Loading 12 ug protein in SDS-PAGE

    QC Testing of P5385
  • Storage Buffer:
  • In 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):
    1. Different concentrations of PAK7 were incubated in a buffer containing 50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at RT for 1 hour.
    2. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at RT.
    3. Fluorescence was then detected using λexc=460±40 nm and λem=528±20 nm filters.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • PAK7
  • Gene Alias:
  • KIAA1264,MGC26232,PAK5
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 7
  • Gene Summary:
  • The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030258,OTTHUMP00000030259,OTTHUMP00000030260,p21(CDKN1A)-activated kinase 7,p21-activated kinase 7,protein kinase PAK5,serine/threonine-protein kinase PAK7
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