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PAK2 (Human) Recombinant ProteinBioActive

  • Catalog # : P5383
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  • Specification
  • Product Description:
  • Human PAK2 kinase domain (Q13177, 227 a.a. - 524 a.a.) partial recombinant protein expressed in Escherichia coli.
  • Sequence:
  • GPHMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR
    The first 4 residues GPHM are from Turbo3C Protease cleavage site. The underlined T is phosphorylated T402.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 33.5
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Concentration:
  • 1 mg/ml
  • Activity:
  • Specific activity: 85,806 pmoles/min/ug
  • Quality Control Testing:
  • Loading 6 ug protein in SDS-PAGE

    QC Testing of P5383
  • Storage Buffer:
  • In 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):
    1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at RT for 1 hour.
    2. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at RT.
    3. Fluorescence was then detected using λexc=460±40 nm and λem=528±20 nm filters.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5062
  • Gene Name:
  • PAK2
  • Gene Alias:
  • PAK65,PAKgamma
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • Gene Summary:
  • The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq
  • Other Designations:
  • S6/H4 kinase,p21 (CDKN1A)-activated kinase 2,p21-activated kinase 2
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