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PAK1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5382
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  • Specification
  • Product Description:
  • Human PAK1 kinase domain (Q13153, 248 a.a. - 545 a.a.) partial recombinant protein expressed in Escherichia coli. The recombinant protein does not have the inhibitory switch domain and has high specific activity.
  • Sequence:
  • GPHMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
    The first 4 residues GPHM are from Turbo3C Protease cleavage site. The underlined T is phosphorylated T423.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 33.7
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Concentration:
  • 1 mg/ml
  • Activity:
  • Specific activity: 32,638 pmoles/min/ug
  • Quality Control Testing:
  • Loading 8 ug protein in SDS-PAGE

    QC Testing of P5382
  • Storage Buffer:
  • In 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):
    1. Different concentrations of PAK1 were incubated in a buffer containing 50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at RT for 1 hour.
    2. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at RT.
    3. Fluorescence was then detected using λexc=460±40 nm and λem=528±20 nm filters.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5058
  • Gene Name:
  • PAK1
  • Gene Alias:
  • MGC130000,MGC130001,PAKalpha
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • Gene Summary:
  • PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • STE20 homolog, yeast,p21-activated kinase 1,p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast),p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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