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Last updated: 2017/7/23

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TurboNucleaseBioActive

  • Catalog # : P5381
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  • Specification
  • Product Description:
  • Serratia macescens extracellular endonuclease expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 27 (monomer)
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Concentration:
  • 250 Units/ul
  • Purity:
  • > 99% (no detectable protease activity)
  • Endotoxin Level:
  • < 0.1 EU/1,000 units as determined by Endo-Safe LAL Assay.
  • Activity:
  • 1 unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 ug of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase.
  • Quality Control Testing:
  • Loading 3 ug protein in SDS-PAGE

    QC Testing of P5381
  • Recommend Usage:
  • Used to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
  • Storage Buffer:
  • In 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% Glycerol
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • 50 ug of salmon sperm DNA was incubated with the indicated units of TurboNuclease at 37°C for 30 minutes in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was check by agarose gel electrophoresis.


  • Biological function:
  • TurboNuclease (aka Benzonase) is a homodimer endonuclease nonspecifically hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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