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Turbo3C Protease BioActive

  • Catalog # : P5380
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  • Specification
  • Product Description:
  • Human rhinovirus (HRV) 3C protease with GST and His tags expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 47
  • Specificity:
  • No non-specific cleavage of target cleavage protein is observed at protease-to-control cleavage protein ratio of 1:10.
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Concentration:
  • 2 mg/ml
  • Purity:
  • > 99% by SDS-PAGE
  • Endotoxin Level:
  • < 5 EU/mg by Charles River EndoSafe-PTS
  • Activity:
  • > 1 Units/ug. 1 Units of Turbo3C (HRV3C) Protease cleaves > 95% of 100 ug of target cleavage protein at 4°C for 16 hours.
  • Recommend Usage:
  • Application that require protein digestion.
  • Storage Buffer:
  • In 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with Turbo3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.


  • Biological function:
  • Turbo3C Protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly.
  • Publication Reference
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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