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Last updated: 2017/4/23

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TurboTEV ProteaseBioActive

  • Catalog # : P5379
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  • Specification
  • Product Description:
  • Tobacco etch virus (TEV) N1a protein catalytic fragment with GST and His tags expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 52
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Concentration:
  • 2 mg/ml
  • Activity:
  • > 10 Units/ug. 1 Unit of TurboTEV Protease cleaves > 85% of 3 ug of control substrate in 1 hour at 30°C.
  • Recommend Usage:
  • Application that require protein digestion.
  • Storage Buffer:
  • In 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TurboTEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 27 kDa and 22 kDa.


  • Biological function:
  • TurboTEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and cleaves between Gln and Gly/Ser.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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