TurboTEV Protease
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Result of activity analysis
Result of activity analysis
A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TurboTEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 27 kDa and 22 kDa.
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Specification
Product Description
Tobacco etch virus (TEV) N1a protein catalytic fragment with GST and His tags expressed in Escherichia coli.
Biological function
TurboTEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and cleaves between Gln and Gly/Ser.
Host
Escherichia coli
Theoretical MW (kDa)
52
Form
Liquid
Preparation Method
Escherichia coli expression system
Concentration
2 mg/ml
Activity
> 10 Units/ug. 1 Unit of TurboTEV Protease cleaves > 85% of 3 ug of control substrate in 1 hour at 30°C.
Recommend Usage
Application that require protein digestion.
Storage Buffer
In 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Enzyme Activity
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