Lyophilized from 10 mM sodium phosphate, pH 7.5 (6 M urea, 2 mM DTT, 1 mM EDTA and 10 mM CH6ClN)
Store at 4°C. This product is stable for at least 3 months. After reconstitution with distilled water, aliquot and store at -20°C. Aliquot to avoid repeated freezing and thawing. Reconstitution: To reconstitute to filaments, after vimentin is dissolved obtain the protofilaments and filament complexes by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and than to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM sodium phosphate, pH 7.4).
This is a highly purified preparation for use as a high molecular weight marker (57 kDa), as a reference standard, or as an antigen for immunogen preparation or various research purposes. For immunization use, further dialyze the solution against PBS (Dulbecco's PBS).