Bovine GFAP native protein from bovine spinal cord.
Theoretical MW (kDa):
Native protein purified from bovine spinal cord
>= 98% by SDS-PAGE
Lyophilized from 10 mM sodium phosphate, pH 7.5 (6 M urea, 1 mM EDTA, 2 mM DTT and 10 mM CH6ClN)
Store at 4°C. This product is stable for at least 3 months. After reconstitution with 80 ul ddH2O water, store at -20°C. Avoid freeze thaw cycles. Reconstitute to filaments by dissolving in 6M urea buffer at concentrations of approximately 0.5 mg/mL. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM sodium chloride, 2 mM dithiothreitol, 10 mM Tris
This is a highly purified protein for use as a high molecular marker (52 kDa), a standard, or an antigen for various research purposes. For immunization, the solution can be further dialyzed against PBS (Dulbecco's PBS).