Jellyfish aequorin native protein from Aequorea sp..
Native protein purified from jellyfish
Lyophilized from buffer salt primarily Sodium EDTA, pH 7.5
Store at -20°C on dry atmosphere. After reconstitution, aliquot and store at 2-8°C. Reconstitution: Reconstitute with calcium free water or other appropriate buffer which is calcium free.
Background luminescence can be caused by some ions including Cd2+, Co2+, Cu2+, lanthanides and Sr2+. Ag+ and Hg+ can cause degradation of aequorin.
Aequorin from Aequorea sp. (jellyfish) has been purified to homogeneity and sequenced from cDNA cloning into different hosts. The Aequorin protein is composed of 189 amino acids formed into a hydrophobic core region where coelenterzine is bound to what is believed to be a peroxidized form since molecular oxygen is not needed for light emission. The protein has a molecular weight of 21.7 kDa, the pI is 4.2-4.9, lambda max 470 nm, and soluble in water at 10 mg/mL.