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Last updated: 2017/3/19

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Aequorin (Aequorea sp.) Native Protein

  • Catalog # : P5340
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  • Specification
  • Product Description:
  • Jellyfish aequorin native protein from Aequorea sp..
  • Host:
  • Jellyfishes
  • Form:
  • Lyophilized
  • Preparation Method:
  • Native protein purified from jellyfish
  • Storage Buffer:
  • Lyophilized from buffer salt primarily Sodium EDTA, pH 7.5
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution, aliquot and store at 2-8°C.
    Reconstitution: Reconstitute with calcium free water or other appropriate buffer which is calcium free.
  • Note:
  • Background luminescence can be caused by some ions including Cd2+, Co2+, Cu2+, lanthanides and Sr2+. Ag+ and Hg+ can cause degradation of aequorin.
  • Background Information:
  • Aequorin from Aequorea sp. (jellyfish) has been purified to homogeneity and sequenced from cDNA cloning into different hosts. The Aequorin protein is composed of 189 amino acids formed into a hydrophobic core region where coelenterzine is bound to what is believed to be a peroxidized form since molecular oxygen is not needed for light emission. The protein has a molecular weight of 21.7 kDa, the pI is 4.2-4.9, lambda max 470 nm, and soluble in water at 10 mg/mL.
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