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Last updated: 2017/3/19

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Tryptase (Human) Native ProteinBioActive

  • Catalog # : P5325
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  • Specification
  • Product Description:
  • Human tryptase native protein from human lung.
  • Host:
  • Human
  • Form:
  • Liquid
  • Preparation Method:
  • Native protein purified from human lung.
  • Concentration:
  • 1 mg/mL
  • Activity:
  • >= 5,000 mU/mg using n-benzoyl-Phe-Val-Arg-pNA as substrate. One unit will hydrolyze one uM of N-benzoyl-Phe-Val-Arg-pNA per minute at 410 nm, pH 8.3, 25°C. The assay uses 0.1 M Tris buffer, pH 8.3, with 0.045 mM substrate and 0.2-0.4 ug of enzyme. E,1%,280nm=30.3.
  • Storage Buffer:
  • In 0.05 M NaOAc, pH 5.0, 1.0 M NaCl, 0.05 mM heparin
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Universal Precautions. Safe laboratory practices should be used when handling this material.
  • Background Information:
  • Tryptase is a mast cell derived serine protease which has been implicated in the pathophysiology of allergic asthma.
  • Applications
  • SDS-PAGE
  • Enzyme Activity
  • Application Image
  • SDS-PAGE
  • Enzyme Activity
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