>= 5,000 mU/mg using n-benzoyl-Phe-Val-Arg-pNA as substrate. One unit will hydrolyze one uM of N-benzoyl-Phe-Val-Arg-pNA per minute at 410 nm, pH 8.3, 25°C. The assay uses 0.1 M Tris buffer, pH 8.3, with 0.045 mM substrate and 0.2-0.4 ug of enzyme. E,1%,280nm=30.3.
In 0.05 M NaOAc, pH 5.0, 1.0 M NaCl, 0.05 mM heparin
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Universal Precautions. Safe laboratory practices should be used when handling this material.
Tryptase is a mast cell derived serine protease which has been implicated in the pathophysiology of allergic asthma.