>=10,000 units per mg protein. One Unit incorporates 1 nmole of tritiated d-TMP into acid precipitable products using poly(A)/oligo(dT)12-18 as the template/primer in 20 minutes at 37°C, pH 8.3.
Reverse Transcriptase Assay; AIDS research; Transcribe mRNA into double stranded cDNA; Used with either single stranded DNA or RNA templates to make probes for use in hybridization experiments; Labeling the termini of DNA fragments with protruding 5' termini.
In 10 mM potassium phosphate, pH 7.4, 1 mM DTT and 20% glycerol
Store at -20°C.
Reverse transcriptases are enzymes encoded in retroviruses viral genome. The enzyme is responsible for transcription of the viral RNA to produce a dsDNA that can be inserted into the host genome.