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Reverse Transcriptase (HIV) Recombinant ProteinBioActive

  • Catalog # : P5324
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  • Specification
  • Product Description:
  • Solution.
  • Host:
  • Escherichia coli
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Activity:
  • >=10,000 units per mg protein. One Unit incorporates 1 nmole of tritiated d-TMP into acid precipitable products using poly(A)/oligo(dT)12-18 as the template/primer in 20 minutes at 37°C, pH 8.3.
  • Recommend Usage:
  • Reverse Transcriptase Assay; AIDS research; Transcribe mRNA into double stranded cDNA; Used with either single stranded DNA or RNA templates to make probes for use in hybridization experiments; Labeling the termini of DNA fragments with protruding 5' termini.
  • Storage Buffer:
  • In 10 mM potassium phosphate, pH 7.4, 1 mM DTT and 20% glycerol
  • Storage Instruction:
  • Store at -20°C.
  • Biological function:
  • Reverse transcriptases are enzymes encoded in retroviruses viral genome. The enzyme is responsible for transcription of the viral RNA to produce a dsDNA that can be inserted into the host genome.
  • Applications
  • Enzyme Activity
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  • Enzyme Activity
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