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Last updated: 2016/12/4

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Nuclease S1 (A. oryzae)BioActive

  • Catalog # : P5323
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  • Specification
  • Product Description:
  • Solution. Activity on native (ds) DNA undetectable under the assay conditions.
  • Host:
  • Fungi
  • Theoretical MW (kDa):
  • ~34
  • Form:
  • Liquid
  • Preparation Method:
  • Native protein chromatographically purified from Aspergillus oryzae
  • Activity:
  • >=100,000 to 500,000 units per ml. One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.
  • Recommend Usage:
  • S1 Nuclease Assay; Eliminate non-annealed polynucleotide tails and hair-pin loops in DNA-RNA or DNA-DNA duplexes in hybridization studies and in genetic recombination experiments.
  • Storage Buffer:
  • In 30 mM sodium acetate, pH 4.6, 50 mM NaCl, 1 mM ZnCl2, and 50% glycerol
  • Storage Instruction:
  • Store at -20°C.
  • Biological function:
  • Nuclease S1 isolated from Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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