Nuclease S1 (A. oryzae)
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Specification
Product Description
Solution. Activity on native (ds) DNA undetectable under the assay conditions.
Biological function
Nuclease S1 isolated from Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA.
Host
Fungi
Theoretical MW (kDa)
~34
Form
Liquid
Preparation Method
Native protein chromatographically purified from Aspergillus oryzae
Activity
>=100,000 to 500,000 units per ml. One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.
Recommend Usage
S1 Nuclease Assay; Eliminate non-annealed polynucleotide tails and hair-pin loops in DNA-RNA or DNA-DNA duplexes in hybridization studies and in genetic recombination experiments.
Storage Buffer
In 30 mM sodium acetate, pH 4.6, 50 mM NaCl, 1 mM ZnCl2, and 50% glycerol
Storage Instruction
Store at -20°C.
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Enzyme Activity
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