Native protein chromatographically purified from S. aureus V8 according to Drapeau et.al, J. Biol. Chem., 247, 6720 (1972)
>=500 units per mg dry weight. One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.
Protease Staph. Aureus (Endoproteinase Glu-C) Assay; Protein sequencing.
Store at 4°C.
Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites.