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Last updated: 2016/12/4

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Protease (S. Aureus), Sequencing Grade

  • Catalog # : P5321
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  • Specification
  • Product Description:
  • Lyophilized.
  • Host:
  • Staphylococcus
  • Theoretical MW (kDa):
  • 27
  • Form:
  • Lyophilized
  • Preparation Method:
  • Native protein chromatographically purified from S. aureus V8 according to Drapeau et.al, J. Biol. Chem., 247, 6720 (1972)
  • Activity:
  • >=500 units per mg dry weight. One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.
  • Recommend Usage:
  • Protease Staph. Aureus (Endoproteinase Glu-C) Assay; Protein sequencing.
  • Storage Instruction:
  • Store at 4°C.
  • Biological function:
  • Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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