Dispase (B. polymyxa)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Lyophilized. Partially purified. Animal Free/AF. Dispase (B. polymyxa) is a non-mammalian animal origin free (AOF) metallo, neutral protease. The mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.
Biological function
Dispase is a mild protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I.
Host
Bacteria
Theoretical MW (kDa)
36
Specificity
Non-specific cleavage of peptide bonds containing leucine and phenylalanine.
pH Optimum
Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0.
Activators
Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2+
Inhibitors
EDTA (1 mM), EGTA, 1-10-phenanthroline and heavy metals
Form
Lyophilized
Preparation Method
Native protein purified from Bacillus polymyxa.
Activity
>= 0.1 units per mg dry weight. One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate.
Recommend Usage
Neutral Protease (Dispase) Assay; Tissue disaggregation and subcultivation; Prevention of unwanted cell clumping; Preparation of cells for culture; Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum; Harvest and transfer of normal, diploid cells and cell lines; Gentle and intact detachment of epidermal cells.
Storage Instruction
Store at 4°C.
Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme Activity
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com