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Last updated: 2016/12/4

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Dispase (B. polymyxa)BioActive

  • Catalog # : P5318
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Lyophilized. Partially purified.
  • Host:
  • Bacteria
  • Theoretical MW (kDa):
  • 36
  • Form:
  • Lyophilized
  • Preparation Method:
  • Native protein purified from Bacillus polymyxa
  • Activity:
  • >=0.1 u/mg dry weight. One Unit releases Folin positive amino acids equivalent to 1 umole tyrosine per minute from casein at 37°C, pH 7.5.
  • Recommend Usage:
  • Neutral Protease (Dispase) Assay; Tissue disaggregation and subcultivation; Prevention of unwanted cell clumping; Preparation of cells for culture; Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum; Harvest and transfer of normal, diploid cells and cell lines; Gentle and intact detachment of epidermal cells.
  • Storage Instruction:
  • Store at 4°C.
  • Biological function:
  • Dispase is a mild protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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