>=0.1 u/mg dry weight. One Unit releases Folin positive amino acids equivalent to 1 umole tyrosine per minute from casein at 37°C, pH 7.5.
Neutral Protease (Dispase) Assay; Tissue disaggregation and subcultivation; Prevention of unwanted cell clumping; Preparation of cells for culture; Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum; Harvest and transfer of normal, diploid cells and cell lines; Gentle and intact detachment of epidermal cells.
Store at 4°C.
Dispase is a mild protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I.