Dispase (B. polymyxa)
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Specification
Product Description
Lyophilized. Purified.
Biological function
Dispase is a mild protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I.
Host
Bacteria
Theoretical MW (kDa)
36
Form
Lyophilized
Preparation Method
Native protein chromatographically purified from Bacillus polymyxa
Activity
>= 4 units per mg dry weight. One Unit releases Folin positive amino acids equivalent to 1 umole tyrosine per minute from casein at 37°C, pH 7.5.
Recommend Usage
Neutral Protease (Dispase) Assay; Tissue disaggregation and subcultivation; Prevention of unwanted cell clumping; Preparation of cells for culture; Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum; Harvest and transfer of normal, diploid cells and cell lines; Gentle and intact detachment of epidermal cells.
Storage Instruction
Store at 4°C.
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Applications
Enzyme Activity
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