Peroxidase (Horseradish), EIA Grade
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Specification
Product Description
Lyophilized. Purified. Single basic isozyme with RZ >2.9.
Biological function
Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuco form of many dyes.
Host
Plants
Theoretical MW (kDa)
40
Form
Lyophilized
Preparation Method
Native protein chromatographically purified from Horseradish Roots
Activity
>=500 units per mg protein. One Unit decomposes 1 umole of H2O2 per minute at 25°C, pH 7.0 using aminoantipyrine and phenol.
Recommend Usage
Immunoconjugation
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Note
The RZ (Reinheitzahl) is the absorbance ratio A403/A275, RZ has been used as an indication of purity.
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Applications
Enzyme Activity
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